Simultaneous Determination of Fe(II) and Fe(III) in Pharmaceutical Samples by Post-Column Derivatization/HPLC
نویسندگان
چکیده
Metal ions can exist in several forms. Ion-exchange plays an important role in modern analytical chemistry. Transition metals can be separated using either anion or cation exchange chromatography, depending on the choice of complexing agent used in the eluent. Iron deficiency is the most common of nutritional anemia in human beings. It is also an essential component of myoglobin, cytochomes, catalase and peroxidase. Iron deficiency can affect the metabolism in muscle independently of the effect of anemia on oxygen delivery. Its deficiency has also been associated with behavioral and learning problems in children and with abnormalities in the catecholamine metabolism. Iron preparations are one of the most commonly used medicaments for the problems arising due to iron deficiency in human beings. An adult male has a requirement of only 13 μg/kg per day (about 1 mg) whereas a menstruating female requires about 21 μg/ kg per day (about 1.4 mg). In the last two trimesters of pregnancy, requirements ∗Corresponding author.
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